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Automated ELISA Workcell

ELISA workcell

ELISA is a commonly used technique that can benefit from automation to allow walkaway processing of plates, freeing up lab workers for other productive tasks.

The OVERLORD™ Automated ELISA workcell provides automation of the key tasks required to run ELISA assays, with the operator input being to add the sample plates to the input stacks and to analyze the data generated by the system.

 

 

ELISA workcell

 

The CAD drawing shows the layout of the EILSA workcell as constructed and installed by Biosero.

Click pictures for larger version.
 

This ELISA workcell consists of:

  • KiNEDx arm with random access multiple stack storage for microplates
  • Beckman Coulter Multimek pipettor
  • Bio-Tek® MicroFill™ Dispenser (quantity 5)
  • Bio-Tek ELx-405™ Washer
  • Molecular Devices SpectraMax® 340 Reader
  • Tables and Mounting Hardware
  • PAA OVERLORD™ Automation Software

Typical order of operations:

  1. Robot moves plate to ELx405 for wash
  2. Robot moves plate to MicroFill and blocking solution is added
  3. Plate returned to stacks for incubate 1 hour at room temp
  4. Robot moves plate to ELx405 for wash
  5. Robot moves plate to MicroFill and blocking solution is added
  6. Plate returned to stacks for incubate 1 hour at room temp
  7. Robot moves plate to ELx405 for wash
  8. Robot moves plate to Multimek and sample is added
  9. Plate returned to stacks for incubate 1 hour at room temp
  10. Robot moves plate to MicroFill and protein solution is added
  11. Plate returned to stacks for incubate 1 hour at room temp
  12. Robot moves plate to MicroFill and secondary antibody is added
  13. Plate returned to stacks for incubate 1 hour at room temp
  14. Robot moves plate to ELx405 for wash
  15. Robot moves plate to MicroFill and development solution is added
  16. Plate incubate for 5 minutes at room temp
  17. Robot moves plate to MicroFill and stop solution is added
  18. Robot moves plate to SpectraMax reader for read step

The OVERLORD software uses static scheduling to manage all of the various movements for optimal throughput of the entire batch of plates.


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