Partners for Automating Science

Automated ELISA Workcell

	ELISA is a commonly used technique that can benefit from automation to allow walkaway processing of plates, freeing up lab workers for other productive tasks. The OVERLORD™ Automated ELISA workcell provides automation of the key tasks required to run ELISA assays, with the operator input being to add the sample plates to the input stacks and to analyze the data generated by the system.
	The CAD drawing shows the layout of the EILSA workcell as constructed and installed by Biosero.
Click pictures for larger version.

This ELISA workcell consists of:

• KiNEDx arm with random access multiple stack storage for microplates
• Beckman Coulter Multimek pipettor
• Bio-Tek® MicroFill™ Dispenser (quantity 5)
• Bio-Tek ELx-405™ Washer
• Molecular Devices SpectraMax® 340 Reader
• Tables and Mounting Hardware
• PAA OVERLORD™ Automation Software

Typical order of operations:

1. Robot moves plate to ELx405 for wash
2. Robot moves plate to MicroFill and blocking solution is added
3. Plate returned to stacks for incubate 1 hour at room temp
4. Robot moves plate to ELx405 for wash
5. Robot moves plate to MicroFill and blocking solution is added
6. Plate returned to stacks for incubate 1 hour at room temp
7. Robot moves plate to ELx405 for wash
8. Robot moves plate to Multimek and sample is added
9. Plate returned to stacks for incubate 1 hour at room temp
10. Robot moves plate to MicroFill and protein solution is added
11. Plate returned to stacks for incubate 1 hour at room temp
12. Robot moves plate to MicroFill and secondary antibody is added
13. Plate returned to stacks for incubate 1 hour at room temp
14. Robot moves plate to ELx405 for wash
15. Robot moves plate to MicroFill and development solution is added
16. Plate incubate for 5 minutes at room temp
17. Robot moves plate to MicroFill and stop solution is added
18. Robot moves plate to SpectraMax reader for read step

 

The OVERLORD software uses static scheduling to manage all of the various movements for optimal throughput of the entire batch of plates.