Partners for Automating Science

Automated ELISA Workcell

	ELISA is a commonly used technique that can benefit from automation to allow walkaway processing of plates, freeing up lab workers for other productive tasks. The OVERLORD™ Automated ELISA workcell provides automation of the key tasks required to run ELISA assays, with the operator input being to add the sample plates to the input stacks and to analyze the data generated by the system.
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This ELISA workcell consists of:

• KiNEDx arm with random access multiple stack storage for microplates
• Hamilton Microlab STAR Liquid Handler
• Molecular Devices AquaMax DW4 Washer/Dispenser
• Tecan Power Washer 384™
• Molecular Devices SpectraMax M2 Reader
• Liconix SGC 40 Incubator Hotel
• Tables and Mounting Hardware
• PAA OVERLORD™ Automation Software

Typical order of operations:

1. Robot moves plate to STAR for addition of standards, QCs, blanks and samples
2. Robot moves plate to shaking hotel incubate 3 hours at room temp
3. Robot moves plate to PW 384 for wash
4. Robot moves plate to DW4 and primary antibody is added
5. Robot moves plate to shaking hotel incubate hour at room temp
6. Robot moves plate to PW 384 for wash
7. Robot moves plate to DW4 and secondary antibody is added
8. Robot moves plate to shaking hotel incubate1 hour at room temp
9. Robot moves plate to PW 384 for wash
10. Robot moves plate to DW4 and substrate is added
11. Robot moves plate to shaking hotel incubate 25 minutes at room temp
12. Robot moves plate to SpectraMax reader for read step

The OVERLORD software uses static scheduling to manage all of the various movements for optimal throughput of the entire batch of plates.